![]() Furthermore, long-read sequencing of native molecules, both DNA and RNA, eliminates amplification bias while preserving base modifications. Long reads can thus improve de novo assembly, mapping certainty, transcript isoform identification, and detection of structural variants. However, natural nucleic acid polymers span eight orders of magnitude in length, and sequencing them in short amplified fragments complicates the task of reconstructing and counting the original molecules. Short-read sequencing is cost-effective, accurate, and supported by a wide range of analysis tools and pipelines. While short-read sequencers such as Illumina’s NovaSeq, HiSeq, NextSeq, and MiSeq instruments BGI’s MGISEQ and BGISEQ models or Thermo Fisher’s Ion Torrent sequencers produce reads of up to 600 bases, long-read sequencing technologies routinely generate reads in excess of 10 kb. Long-read sequencing, or third-generation sequencing, offers a number of advantages over short-read sequencing.
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